Protein Engineering by Semisynthesis

Author: Carmichael J.A. Wallace
Publisher: CRC Press
ISBN: 0849347270
Release Date: 1999-09-29
Genre: Medical

As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features

Molecular Basis of Insulin Action

Author: Michael P. Czech
Publisher: Springer Science & Business Media
ISBN: 9781468448740
Release Date: 2013-11-11
Genre: Science

One day, in a moment of weakness, I fell prey to the temptation to organize and edit this volume on the mechanism of insulin action. The major reason for attempting to resist, of course, is the amazing speed at which advances are being made in this field. The usefulness of books such as this is often quickly compromised by new findings obtained during and just after publication. Happily for the contributors to this volume and myself, this unfortunate fate does not appear to be in store for us. New and important findings will undoubtedly continue to flow in this field during the next few years, but I believe this will increase rather than decrease the usefulness of this volume. As a matter of fact, as we go to press, I am delighted both that I was tempted and that I failed to resist. There are two basic reasons for my enthusiasm about this book, and they both relate to this issue of timeliness. First, each of the contributors has had an opportunity to update the scientific content of the various chapters only a few months before actual publication of this volume. The material presented in this volume is, at publication, contemporary with the current original literature. This volume thus provides an ex cellent framework for assessing new discoveries in this field for some time to come.

Biochemical Actions of Hormones

Author: Gerald Litwack
Publisher: Elsevier
ISBN: 9780323146166
Release Date: 2012-12-02
Genre: Science

Biochemical Actions of Hormones, Volume II is a 12-chapter text that surveys the significant developments toward understanding the primary effects of hormones in cellular receptors at the molecular level. This book starts with an overview of the genetic regulation by hormones and the role of cyclic adenosine monophosphate in hormonal response. These topics are followed by discussions on the hormone-dependent molecular mechanisms, which appear to participate in the regulation of cell proliferation and differentiation in this model system, as well as how multiple hormonal signals may serve to integrate a large number of complex regulatory mechanisms. A chapter deals with the comparative aspects of the physiology and biochemistry of insulin, with an emphasis on the molecular mode of action of insulin on metabolic processes. The remaining chapters deal with the physiology, mechanism of action, and biological effects of various hormones, including catecholamines, glucocorticoids, estrogens, progestins, gonadotropins, prolactin, adrenocorticotropic hormone, 25-hydroxycholecalciferol, and insect hormones. Endocrinologists will find this book invaluable.

Human Insulin

Author: D.R. Owens
Publisher: Springer Science & Business Media
ISBN: 9789400941618
Release Date: 2012-12-06
Genre: Medical

Since insulin became available for the treatment of diabetes in 1922 a number of major advances have been made, which include the modification of insulin to vary its timing of action, its purification, and latterly, the production of human insulin. Human insulin in quantities sufficiently large for therapy has been made available by two techniques developed in parallel during the late 1970s. These involve either (i) formulation in E. coli bacteria suitably encoded by DNA recombinant methods of the A- and B-chains of human insulin followed by a chain combination reaction ('biosynthetic' human insulin) or (ii) enzymatic conversion (transpeptidation) of porcine insulin brought to react with a threonine ester by porcine trypsin in a mixture of water and organic solvents, yielding human insulin ('semi-synthetic' human insulin). This book includes the first clinical-pharmacological studies of each of the highly purified 'semi-synthetic' human insulin preparations: Actrapid ® HM; Monotard® HM; Protaphane® HM; Actraphane® HM; and Ultratard® HM (Novo Industri A/S, Copenhagen). The preliminary studies established their safety and efficacy relative to their porcine and bovine counterparts emphasising the relevance of species and formulation on the pharmacokinetics and biological responses to insulin. Additional investigations with human insulin demonstrated the influence of insulin concentration, site of administration, the addition of aprotinin to insulin and the mixing of 'short-' and 'intermediate-acting' formulations on insulin 'bioavailability'. Examination of the 'within' and 'between' subject day-to-day variation in absorption and the effect of subcutaneous insulin also demonstrates the dominating influence of insulin responsiveness.

Progress of Lens Biochemistry Research Volume in honour of Prof Dr med J Nordmann

Author: O. Hockwin
Publisher: Springer Science & Business Media
ISBN: 9789401015714
Release Date: 2012-12-06
Genre: Medical

On August 25,1976 Prof. Dr. med. JEAN NORDMANN, Emeritus Professor of Strasbourg University, will celebrate his 80th anniversary. The task of paying tribute to Professor NORDMANN's many services to ophthalmology in a way worthy of the occasion is so difficult that we decided to concentrate on the focal point of his life's work. In this issue scientists engaged in lens research from allover the world offer reports of their latest findings together with their best wishes and felicitations as a birthday gift to the Nestor of lens research. They thereby express their gratitude to Professor NORDMANN, who, with the lifelong in defatigable enthusiasm of a scientist, has most successfully persued the prob lems of lens metabolism. It is our sincerest hope that we may look forward to many more years in which to take advantage of Professor NORDMANN's superior knowledge. Among the contributions to this festive donation we miss the works of some authors who have always held Professor NORDMANN's special inter est. The editor has received several letters of longstanding friends who would have liked to submit a manuscript but had been unable to meet the deadline: Professor NORDMANN may rest assured that they, too, belong to the great number of congratulators from our field of research. We have been able to make this dedication thanks to Professor HENKES who readily agreed to publish our contributions in Documenta Ophthal mologica. Dr. Junk publishers have done their utmost to ensure prompt publication.

Characterization regulation and interactions within the protease web

Author: Viktor Magdolen
Publisher: Walter de Gruyter
ISBN: 9783110260373
Release Date: 2013-01-01
Genre: Science

The KLK proteins and their encoding genes are increasingly attracting attention among scientists and clinicians worldwide as they represent interesting and functionally distinct biomarkers both under physiological and pathophysiological conditions. This volume on kallikrein-related peptidases (KLKs) reviews the characterization, regulation, and interactions of these proteases within the protease web.

Protein Precursors Advances in Research and Application 2013 Edition

Publisher: ScholarlyEditions
ISBN: 9781481679602
Release Date: 2013-06-21
Genre: Medical

Protein Precursors—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Proinsulin. The editors have built Protein Precursors—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Proinsulin in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Protein Precursors—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at

Endocrinology Adult and Pediatric E Book

Author: J. Larry Jameson
Publisher: Elsevier Health Sciences
ISBN: 9780323321952
Release Date: 2015-02-25
Genre: Medical

Considered the definitive source in its field for over 35 years, Endocrinology: Adult and Pediatric, has been thoroughly updated to reflect today's recent advances in adult and pediatric endocrinology. Unique perspectives from a team of trusted, world-renowned experts ensure this medical reference book remains the most highly-regarded text in the field. Make the best clinical decisions with an enhanced emphasis on evidence-based practice and expert opinions on treatment strategies. Zero in on the most relevant and useful references with the aid of a more focused, concise bibliography. Locate information quickly, while still getting the complete coverage you expect. Expanded coverage for key topics such as pediatric endocrinology and obesity mechanisms and treatment, in addition to today's hot topics in endocrinology, including endocrine disruptors, bariatric surgery, androgen deficiency, genetic causes of obesity, endocrine rhythms, and the use of tyrosine kinase inhibitors in thyroid cancer. New content addressing the latest advances in testosterone and estrogen replacement, as well as the new causes of calcium and phosphate disorders, new molecular causes of endocrine cancers, new genetic causes of reproductive disorders, and more. Updated clinical guidelines for diabetes, lipid disorders, obesity management, osteoporosis, and more, as well as essential treatment updates for the medical management of acromegaly, Cushing's Disease, hypercalcemia, and diabetes mellitus. New Key Points provide snapshots of what to expect in each chapter, or serve as a refresher of what you just read. Consult this title on your favorite e-reader.


Author: R. D. G. Leslie
Publisher: Cambridge University Press
ISBN: 0521450292
Release Date: 1995-03-09
Genre: Medical

This significant new publication provides a summary and overview of the most recent scientific advances in diabetes research, and highlights the role of these developments in advancing clinical practice. Diabetes is one of the major causes of morbidity and mortality in the developed world, and its prevalence elsewhere is on the increase. It affects in excess of 100 million people worldwide. Recent scientific advances have clarified our understanding of the causes and consequences of this devastating disease. These scientific developments are playing an increasingly important role in clinical practice and could well lead the way to the prevention of complications and even a cure for the disease. An international and authoritative team of authors draws together this knowledge and the most recent developments to provide an up to date account which will benefit researchers and clinicians.

Enzymes in Organic Synthesis

Author: Ruth Porter
Publisher: John Wiley & Sons
ISBN: 9780470718629
Release Date: 2009-09-14
Genre: Science

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.