Lipid Metabolism in Signaling Systems

Author: John N. Fain
Publisher: Academic Press
ISBN: 9781483288321
Release Date: 2013-10-22
Genre: Science

Many of the phospholipases and even the receptor for inositol 1,4,5-trisphosphate have recently been cloned and sequenced and play an important role in neurotransmission. Methods developed in recent years for the study of the enzymes and intermediates involved in the regulation of neuronal signal transduction involving phospholipid turnover are presented in this volume. Techniques for examining the role of phosphoinositides in signal transduction Techniques related to protein kinase C Analysis of inositol phosphates Techniques to analyze phospholipid turnover in the brain

Inositol Phospholipid Metabolism and Phosphatidyl Inositol Kinases

Author: Arnis Kuksis
Publisher: Elsevier
ISBN: 9780444513212
Release Date: 2003
Genre: Science

Offers a review of developments along with tried and tested methods for isolation, resolution and quantification of inositol phospholipids and inositol polyphosphates in both cells and tissues. This book includes detailed methodology for identification of molecular species of inositol phospholipids, including their phosphates and glycans.

Neurobiology of Cytokines

Author: Errol B. De Souza
Publisher: Academic Press
ISBN: 9781483217550
Release Date: 2013-10-22
Genre: Health & Fitness

Neurobiology of Cytokines, Part A provides an overview of the effects of cytokines in the brain and in the endocrine system. The book discusses the general aspects of cytokines, including the endogenous agonists and antagonists, their receptors, their second messengers, and transport mechanisms for cytokines across the blood-brain barrier. The text also describes the anatomical localization of cytokines, cytokine receptors, and their respective mRNA in brain and in endocrine tissues. The methods for evaluating the in vivo as well as in vitro actions of cytokines on hormone secretion are also considered. The book further tackles the synthesis and release of cytokines and their central nervous system actions; and the methodology for studying the role of cytokines in human neuropathological conditions.

Neurobiology of Steroids

Author: E. Ronald de Kloet
Publisher: Academic Press
ISBN: 9781483288369
Release Date: 2013-10-22
Genre: Science

Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions. Comprehensive protocols included for the study of Steroid kinetics and metabolism Steroid receptors Molecular and cellular effects of steroids Steroid effects on integrated systems

Providing Pharmacological Access to the Brain

Author: Thomas R. J. Flanagan
Publisher:
ISBN: UVA:X002540078
Release Date: 1994
Genre: Blood-brain barrier

This volume focuses on contemporary approaches for delivering experimental and therapeutic agents into the brain. The contributions provide methodological details that are typically not available in the literature. Subtleties and shortcuts critical to each procedure are included to facilitate their use by both the experienced researcher and novice. Highlights * Polymeric, cellular, and molecular drug delivery * Neuropharmacology * Blood-brain barrier * Central nervous system

PCR in Neuroscience

Author: Gobinda Sarkar
Publisher:
ISBN: 0121852962
Release Date: 1995
Genre: Neurosciences

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Key Features * Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) * Complete retrieval of novel expressed genes by PCR without screening a library * Quantitation by PCR * Mutagenesis by PCR * PCR in AIDS research * Simple and effective protocols for PCR on archival specimens.

Pulsatility in Neuroendocrine Systems

Author: Jon E. Levine
Publisher:
ISBN: 012185289X
Release Date: 1994-01-01
Genre: Science

Pulsatility is now recognized as a nearly ubiquitous functional feature of neuroendocrine systems. This volume presents a comprehensive guide to the established and emerging technologies being used to study the perplexing phenomenon of pulsatility. Molecular, cellular, physiological, and mathematical approaches are described in detail. Comprehensive protocols included for the study of * In vitro methods for studying neuroendocrine pulsatility * In vivo sampling and recording procedures for monitoring pulsatility in several species * Improved quantitative and analytical methods for the study of hormone pulsatility